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Sample GSM66298 Query DataSets for GSM66298
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu407 NS4 Sgu407 YPD Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu407 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu407 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu407 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu407 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.68 486 78 778 150 0
2 0.14 1014 186 918 358 0
3 -1.18 1677 81 3801 177 0
4 -0.67 1266 181 2012 385 0
5 -1.00 8815 74 17616 154 0
6 -0.60 1846 177 2798 339 0
7 -0.79 519 80 900 183 0
8 -0.61 804 222 1227 461 0
9 -1.01 682 74 1369 159 0
10 -0.64 1661 286 2583 599 0
11 0.17 7946 96 7047 223 0
12 -0.27 1100 282 1330 598 0
13 -0.46 1520 155 2084 337 0
14 -0.82 591 342 1042 706 -50
15 -0.81 1799 225 3152 483 0
16 -0.82 681 142 1206 309 0
17 -0.85 427 87 771 175 0
18 -0.45 1485 113 2035 250 0
19 -0.20 1117 79 1279 171 0
20 -0.30 1986 177 2442 375 0

Total number of rows: 6218

Table truncated, full table size 179 Kbytes.




Supplementary data files not provided

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