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Sample GSM66301 Query DataSets for GSM66301
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu407 SWM Sgu407 NS4 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu407 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu407 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu407 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu407 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.36 108 37 278 78 0
2 -0.96 149 46 290 85 0
3 -0.94 2523 40 4843 82 0
4 -1.22 286 47 664 81 0
5 -1.63 2081 44 6424 93 0
6 -0.78 578 44 995 84 0
7 -1.22 112 43 260 87 0
8 -1.18 115 42 260 83 0
9 -1.42 105 42 281 86 0
10 -0.66 132 45 209 78 0
11 0.11 1197 43 1112 85 0
12 -0.71 116 43 190 74 0
13 -0.91 137 42 258 84 0
14 -0.98 128 42 253 74 0
15 -1.33 269 44 675 86 0
16 -1.07 91 43 191 71 0
17 -1.48 53 39 148 79 0
18 -1.00 278 45 556 85 0
19 -0.78 151 42 259 84 0
20 -1.08 201 45 426 82 0

Total number of rows: 6218

Table truncated, full table size 162 Kbytes.




Supplementary data files not provided

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