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Sample GSM66303 Query DataSets for GSM66303
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu407 YPD Sgu407 NS24
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu407 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu407 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu407 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu407 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.57 54 58 80 67 0
2 -0.53 77 60 111 71 0
3 -0.63 2137 60 3312 66 0
4 -0.27 69 55 83 71 0
5 -0.70 2957 62 4791 70 0
6 -0.52 525 60 753 70 0
7 -1.68 128 60 409 71 0
8 -1.43 114 56 308 70 0
9 -0.89 117 64 217 70 0
10 -1.21 157 76 362 89 0
11 -0.55 528 64 775 67 0
12 -1.02 134 73 271 90 0
13 -1.63 127 64 394 68 0
14 -1.21 150 59 348 70 0
15 -0.97 368 63 722 65 0
16 -0.02 80 65 81 71 -50
17 -0.60 73 58 111 68 0
18 -0.39 229 60 300 74 0
19 -1.08 136 57 288 68 0
20 -0.59 332 59 500 72 0

Total number of rows: 6218

Table truncated, full table size 163 Kbytes.




Supplementary data files not provided

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