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Sample GSM66312 Query DataSets for GSM66312
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu421 SWM Sgu421 NS24 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu421 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu421 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.07 342 99 360 162 0
2 -0.69 325 72 524 148 0
3 2.05 14047 91 3400 179 0
4 0.56 451 74 305 144 0
5 1.01 11697 87 5799 169 0
6 1.67 759 76 238 131 0
7 -0.92 303 84 572 163 0
8 0.15 275 72 248 122 0
9 -0.50 272 124 386 195 0
10 -0.54 204 70 297 146 0
11 -0.58 1496 105 2241 193 0
12 -1.01 168 68 339 132 0
13 -0.36 467 102 601 198 0
14 -0.19 208 72 237 127 0
15 0.08 911 89 862 189 0
16 0.39 378 68 288 121 0
17 -0.83 156 96 277 166 0
18 0.25 764 72 642 147 0
19 -0.52 478 90 684 182 0
20 0.47 431 76 311 146 0

Total number of rows: 6218

Table truncated, full table size 168 Kbytes.




Supplementary data files not provided

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