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Sample GSM66313 Query DataSets for GSM66313
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu421 SWM Sgu421 NS24 Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu421 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu421 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 0.11 701 99 649 165 0
2 -1.01 1017 124 2050 240 0
3 1.96 18227 130 4686 201 0
4 0.43 1898 125 1409 239 0
5 0.92 27826 153 14701 225 0
6 1.80 4495 140 1292 247 0
7 -0.93 859 128 1633 228 0
8 -0.15 762 133 848 224 0
9 -0.11 864 141 931 198 0
10 -0.18 1580 142 1796 231 0
11 -0.38 6683 131 8676 194 0
12 -1.00 1142 141 2288 221 0
13 -0.12 1803 121 1964 195 0
14 0.01 1361 144 1354 229 0
15 0.47 5772 123 4177 199 0
16 -0.09 1082 138 1151 208 0
17 -0.82 197 102 347 162 0
18 0.11 2509 117 2324 236 0
19 -0.18 811 132 916 208 0
20 0.38 996 126 766 226 0

Total number of rows: 6218

Table truncated, full table size 181 Kbytes.




Supplementary data files not provided

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