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Sample GSM66314 Query DataSets for GSM66314
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu421 SWM Sgu421 NS4 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu421 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu421 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.97 130 51 254 127 0
2 -1.05 119 55 246 129 0
3 3.61 3138 50 257 125 0
4 -0.15 205 53 227 129 0
5 3.22 3076 51 329 125 0
6 0.78 407 52 237 127 0
7 -1.25 112 52 267 125 0
8 -0.88 127 51 233 126 0
9 -1.41 89 49 236 119 0
10 -1.29 121 57 296 133 0
11 1.74 872 52 261 124 0
12 -1.46 107 54 295 131 0
13 -0.89 110 52 204 127 0
14 -0.95 129 53 250 128 0
15 -0.18 236 52 268 128 0
16 -0.70 149 52 242 121 0
17 -1.87 73 49 266 125 0
18 -0.40 203 56 268 129 0
19 -0.96 122 50 238 123 0
20 -0.18 198 53 225 126 0

Total number of rows: 6218

Table truncated, full table size 166 Kbytes.




Supplementary data files not provided

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