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Sample GSM66315 Query DataSets for GSM66315
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu421 SWM Sgu421 NS4 Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu421 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu421 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.15 67 34 149 70 0
2 -0.94 71 44 136 77 0
3 3.32 1409 36 141 71 0
4 -0.36 98 44 126 76 0
5 3.01 1336 39 166 73 0
6 0.11 158 41 146 75 0
7 -1.03 64 38 131 73 0
8 -0.98 64 39 126 75 0
9 -1.14 63 35 139 66 0
10 -0.98 78 44 154 77 0
11 1.85 436 37 121 68 0
12 -0.82 67 43 118 74 0
13 -0.84 85 39 152 73 0
14 -0.58 74 40 111 71 0
15 -0.09 128 40 136 71 0
16 -0.53 84 38 121 74 0
17 -1.63 46 34 142 70 0
18 -0.37 104 43 134 76 0
19 -0.93 71 37 135 70 0
20 -0.24 116 43 137 75 0

Total number of rows: 6218

Table truncated, full table size 157 Kbytes.




Supplementary data files not provided

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