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Sample GSM66316 Query DataSets for GSM66316
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu421 YPD BY4743 YPD Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu421 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.42 588 52 786 76 0
2 -0.37 1315 39 1695 64 0
3 0.61 16825 57 11054 78 0
4 0.58 1620 42 1086 67 0
5 0.33 29975 57 23857 80 0
6 -0.29 4986 48 6108 71 0
7 -1.66 757 54 2393 74 0
8 -0.57 1036 58 1541 80 0
9 -0.90 667 37 1247 56 0
10 -0.36 800 49 1024 68 0
11 0.57 15024 44 10113 57 0
12 0.02 1178 37 1163 54 0
13 -0.20 1689 45 1942 63 0
14 -0.48 997 48 1388 67 0
15 -0.31 4009 50 4982 66 0
16 0.02 1029 48 1017 66 0
17 -0.37 142 53 183 79 0
18 -0.03 3759 40 3851 64 0
19 0.20 1556 54 1356 79 0
20 0.09 2477 41 2333 65 0

Total number of rows: 6218

Table truncated, full table size 171 Kbytes.




Supplementary data files not provided

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