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Sample GSM66318 Query DataSets for GSM66318
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu421 YPD BY4743 YPD Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu421 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.61 473 35 721 58 0
2 -0.36 1319 41 1697 66 0
3 0.39 16431 41 12575 65 0
4 0.44 1614 52 1192 84 0
5 0.01 28895 44 28759 68 0
6 -0.27 5445 55 6560 86 0
7 -1.87 751 40 2753 65 0
8 -0.62 1118 64 1719 98 0
9 -0.89 994 53 1837 80 0
10 -0.46 609 56 837 82 0
11 0.53 18612 58 12855 84 0
12 -0.12 1093 53 1186 81 0
13 -0.23 2468 63 2892 89 0
14 -0.53 898 48 1299 73 0
15 -0.34 4447 59 5626 84 0
16 -0.07 985 49 1033 71 0
17 -0.64 109 37 170 59 0
18 -0.04 4103 45 4219 72 0
19 -0.02 1526 42 1545 66 0
20 -0.10 2377 53 2556 85 0

Total number of rows: 6218

Table truncated, full table size 171 Kbytes.




Supplementary data files not provided

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