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Sample GSM66319 Query DataSets for GSM66319
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu421 YPD BY4743 YPD Replicate2_Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu421 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.47 54 31 75 41 0
2 0.05 435 31 421 39 0
3 -0.26 802 34 960 46 0
4 -0.57 74 35 110 47 0
5 0.43 1770 39 1313 50 0
6 0.03 652 35 638 47 0
7 0.24 1463 36 1239 51 0
8 -0.65 819 36 1284 48 0
9 0.09 1470 34 1380 51 0
10 0.16 1472 44 1320 65 0
11 0.38 1405 34 1076 50 0
12 0.08 1029 34 971 48 0
13 -0.15 1456 33 1619 48 0
14 0.10 923 34 859 48 0
15 0.64 2770 34 1781 47 0
16 -1.04 65 31 134 44 0
17 0.41 2737 33 2067 43 0
18 -0.09 1036 33 1106 45 0
19 -0.07 1399 37 1465 47 0
20 -0.30 998 36 1232 47 0

Total number of rows: 6218

Table truncated, full table size 165 Kbytes.




Supplementary data files not provided

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