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Sample GSM66320 Query DataSets for GSM66320
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu421 YPD Sgu421 SWM
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu421 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu421 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.38 60 46 78 68 0
2 -0.38 160 46 208 67 0
3 -0.48 2624 45 3661 68 0
4 -0.40 99 47 131 67 0
5 0.14 8483 47 7701 70 0
6 -0.12 2189 46 2387 69 0
7 0.08 469 46 445 70 0
8 -0.40 367 46 483 68 0
9 -0.04 263 52 271 71 0
10 -0.55 298 49 435 68 0
11 1.00 3198 47 1596 67 0
12 -0.11 342 49 369 67 0
13 0.33 579 47 461 68 0
14 0.16 464 48 414 66 0
15 0.50 1632 48 1152 69 0
16 -0.39 78 47 102 65 0
17 -0.76 65 45 110 68 0
18 -0.44 745 47 1013 67 0
19 -0.18 283 46 320 69 0
20 0.07 920 46 878 66 0

Total number of rows: 6218

Table truncated, full table size 164 Kbytes.




Supplementary data files not provided

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