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Sample GSM66325 Query DataSets for GSM66325
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu52 NS4 Sgu52 YPD Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu52 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu52 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.48 4746 41 13257 68 0
2 -2.57 227 41 1351 65 0
3 -2.55 116 42 681 74 0
4 -1.29 146 44 358 64 0
5 -3.14 1245 44 10988 72 0
6 -1.90 191 45 713 68 0
7 -2.82 1378 47 9762 77 0
8 -2.47 462 46 2563 77 0
9 -1.87 716 54 2625 86 0
10 -1.01 76 54 153 94 0
11 -2.72 511 52 3369 88 0
12 -1.42 206 57 551 98 0
13 -1.56 1248 51 3689 86 0
14 -1.63 491 51 1517 96 0
15 -1.25 2557 50 6074 92 0
16 -1.24 2306 53 5460 92 0
17 -2.29 248 38 1214 67 0
18 -1.50 4705 39 13286 66 0
19 -1.81 336 42 1182 74 0
20 -2.15 324 43 1434 65 0

Total number of rows: 6218

Table truncated, full table size 168 Kbytes.




Supplementary data files not provided

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