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Sample GSM66326 Query DataSets for GSM66326
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu52 NS4 Sgu52 YPD Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu52 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu52 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.45 3801 42 10364 71 0
2 -2.31 177 39 875 70 0
3 -2.21 90 44 416 75 0
4 -1.52 89 44 255 77 0
5 -3.38 638 46 6648 74 0
6 -1.72 140 44 460 72 0
7 -2.65 1047 47 6562 76 0
8 -2.64 253 47 1577 78 0
9 -1.93 434 54 1656 82 0
10 -1.44 759 52 2065 86 0
11 -2.88 256 51 1883 82 0
12 -1.53 86 50 248 83 0
13 -2.41 415 50 2211 83 0
14 -1.95 203 49 787 83 0
15 -1.47 1975 49 5479 79 0
16 -1.60 1110 45 3356 80 0
17 -2.22 200 42 935 70 0
18 -1.84 3463 40 12392 71 0
19 -1.67 227 45 724 76 0
20 -2.11 374 44 1620 74 0

Total number of rows: 6218

Table truncated, full table size 167 Kbytes.




Supplementary data files not provided

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