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Sample GSM66329 Query DataSets for GSM66329
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu52 SWM Sgu52 NS4 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu52 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu52 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.08 262 44 552 76 0
2 -0.59 664 56 1002 93 0
3 -1.32 3620 44 9043 78 0
4 -1.31 554 52 1369 94 0
5 0.91 22725 51 12076 86 0
6 -1.47 556 53 1544 92 0
7 0.16 1052 48 942 84 0
8 -1.23 228 50 533 87 0
9 -0.79 487 47 844 80 0
10 -0.53 368 58 532 98 0
11 -2.14 1448 49 6396 86 0
12 -1.18 325 54 737 86 0
13 -0.33 826 51 1041 92 0
14 -0.82 313 52 553 86 0
15 -0.67 1701 54 2713 95 0
16 -0.78 198 50 341 85 0
17 -0.10 173 43 185 74 0
18 -0.55 880 55 1291 92 0
19 -0.76 375 43 635 77 0
20 -0.93 455 52 865 91 0

Total number of rows: 6218

Table truncated, full table size 167 Kbytes.




Supplementary data files not provided

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