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Sample GSM66331 Query DataSets for GSM66331
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu52 YPD Sgu52 NS24 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu52 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu52 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.77 34 26 116 60 0
2 -2.57 291 32 1728 99 0
3 0.52 5722 31 3999 83 0
4 -1.75 380 30 1279 93 0
5 -0.10 12013 40 12875 101 0
6 0.15 837 30 755 91 0
7 -3.76 147 33 1988 92 0
8 -2.08 266 32 1127 96 0
9 -1.62 422 31 1294 78 0
10 -1.97 77 27 302 82 0
11 2.92 4480 32 592 88 0
12 -1.88 85 28 313 84 0
13 -1.62 398 32 1221 93 0
14 -1.82 76 29 268 82 0
15 -1.88 962 31 3553 95 0
16 -2.23 158 27 741 77 0
17 -1.65 47 29 148 76 0
18 -0.80 763 30 1332 91 0
19 -1.35 256 31 652 83 0
20 -1.70 658 30 2139 93 0

Total number of rows: 6218

Table truncated, full table size 166 Kbytes.




Supplementary data files not provided

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