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Sample GSM66333 Query DataSets for GSM66333
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu52 YPD Sgu52 SWM Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu52 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu52 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.71 473 33 776 67 0
2 -0.38 1192 65 1549 127 0
3 -0.28 7999 35 9736 73 0
4 -0.63 501 49 777 102 0
5 0.53 23929 43 16525 87 0
6 -0.27 3226 55 3886 109 0
7 -0.26 478 37 573 85 0
8 -0.02 768 53 779 105 0
9 -0.08 718 36 757 70 0
10 -0.24 788 51 933 105 0
11 0.51 7495 36 5277 71 0
12 0.31 1141 46 921 96 0
13 -0.40 1173 36 1549 76 0
14 0.42 899 44 670 92 0
15 -0.31 3070 36 3798 80 0
16 -0.69 887 44 1430 90 0
17 -0.79 95 34 164 69 0
18 -0.04 3443 59 3528 117 0
19 0.15 814 35 733 73 0
20 -0.37 428 50 553 104 0

Total number of rows: 6218

Table truncated, full table size 170 Kbytes.




Supplementary data files not provided

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