|
| Status |
Public on Apr 13, 2011 |
| Title |
Healthy EPCs/CACs, non-IFNa treated, 102 |
| Sample type |
RNA |
| |
|
| Source name |
Healthy EPCs/CACS not incubated with IFNa
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: endothelial progenitor cells (EPCs)/myeloid circulating angiogenic cells (CACs)
disease state: healthy
treatment: untreated
|
| Treatment protocol |
Cells were incubated in the presence or absence of IFNa for 6 hours prior to RNA harvesting.
|
| Growth protocol |
PBMCs containing EPCs and CACs were cultured in endothelial cell-specific enrichment medium on fibronectin-coated wells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Tri-pure (Roche), and further purified and concentrated using an Rneasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
|
| Description |
Ctrl_NT_102
Gene expression data from healthy EPCs/CACs not-incubated with IFNa.
|
| Data processing |
The data were analyzed using the GenePattern pipeline: the RMA method and Human Entrez Gene custom CDF annotation version 10 was used for the normalization. Healthy EPCs, lupus EPCs and healthy bone marrow EPCs were normalized separately.
|
| |
|
| Submission date |
Jan 28, 2011 |
| Last update date |
Apr 13, 2011 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL11670 |
| Series (2) |
| GSE23203 |
Expression data from human healthy and lupus EPCs/CACs, and healthy CD133+ bone marrow EPCs |
| GSE26949 |
Expression data from human healthy EPCs/CACs |
|
