|
| Status |
Public on Jun 22, 2011 |
| Title |
Lupus neutrophil, 009 |
| Sample type |
RNA |
| |
|
| Source name |
Neutrophil from lupus patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: neutrophils
disease state: lupus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Tri-pure (Roche), and further purified and concentrated using an Rneasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
|
| Description |
LupusNeutro009
Gene expression data from lupus neutrophils.
|
| Data processing |
The data were analyzed using the GenePattern pipeline: the RMA method and Human Entrez Gene custom CDF annotation version 10 were used for the normalization, and Combat for batch correction.
|
| |
|
| Submission date |
Jan 31, 2011 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL11670 |
| Series (1) |
| GSE26975 |
Human lupus netting neutrophils induce endothelial damage, infiltrate tissues and expose immunostimulatory molecules |
|
| Relations |
| Reanalyzed by |
GSE86362 |
