|
| Status |
Public on Dec 16, 2022 |
| Title |
Mouse_Liver_10x_SingleCell_KO2 |
| Sample type |
SRA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
genotype: SIRT7 KO
age: 5-6 mo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hepatocytes and non-parenchymal cells (NPCs) were isolated by a two-step collagenase perfusion method (Tabula Muris et al., 2018). Cell viability was confirmed by trypan blue exclusion.
3000 hepatocytes and 3000 NPCs were mixed and used directly for scRNA-seq analysis using 10X Genomics Chromium Single-Cell 3’ according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing, and gene counting were made using the Cell Ranger software v6.0.0 (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger)
Assembly: mm10
Supplementary files format and content: Comma-separated values file
|
| |
|
| Submission date |
Oct 30, 2022 |
| Last update date |
Dec 16, 2022 |
| Contact name |
Danica Chen |
| Organization name |
University of California, Berkeley
|
| Street address |
335 Morgan Hall
|
| City |
Berkeley |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platform ID |
GPL30172 |
| Series (2) |
|
| Relations |
| BioSample |
SAMN31525362 |
| SRA |
SRX18077962 |
