|
| Status |
Public on Jan 24, 2023 |
| Title |
DSP-0509-2 |
| Sample type |
RNA |
| |
|
| Source name |
CT26 tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: CT26 tumor
treatment: DSP-0509
|
| Treatment protocol |
DSP-0509 was dosed with 5 mg/kg i.v. and anti PD-1 antibody was dosed with 200ug/mouse. Tumors were resected 4 days after dosing.
|
| Growth protocol |
1x10^6 cells of CT26 were inoculated into dorsal frank in Balb/c mouse. When tumor volume reached 130-170mm^3, mice were assigned to each group.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA from in vivo CT26 tumors were extracted by using Maxwell RSC simply RNA tissue kit.
|
| Label |
n/a
|
| Label protocol |
RNA was lebeled / hybridized with gene specific fluorescent probe using nCounter PanCancer Mouse Immune Profiling kit as descrived in protocol by vendor
|
| |
|
| Hybridization protocol |
RNA was lebeled / hybridized with gene specific fluorescent probe using nCounter PanCancer Mouse Immune Profiling kit as descrived in protocol by vendor
|
| Scan protocol |
Fluorescent signal was scanned by using nCounter MAX Analysis system as described in protocol by vendor
|
| Data processing |
Raw data was normalized by normalization module within nCounter Advanced Analysis 2.0. Probes for normalization were selected based on algolizum within normalization module
|
| |
|
| Submission date |
Oct 31, 2022 |
| Last update date |
Jan 24, 2023 |
| Contact name |
Yosuke Ota |
| E-mail(s) |
se3pf30@gmail.com
|
| Organization name |
Sumitomo Pharma
|
| Department |
Cancer Research Unit
|
| Street address |
3-1-98 Kasugade-naka konohanaku
|
| City |
Osaka |
| State/province |
Osaka |
| ZIP/Postal code |
554-0022 |
| Country |
Japan |
| |
|
| Platform ID |
GPL30298 |
| Series (1) |
| GSE216890 |
nCounter PanCancer mouse immune profiling in CT26-bearing mouse tumor treated with DSP-0509 combined with anti PD-1 antibody |
|
