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Sample GSM67370 Query DataSets for GSM67370
Status Public on Sep 26, 2005
Title del337TR replicate 2
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics deletion in 337TR
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description deletion in 337TR
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 262.1 P 0.000857
AFFX-BioB-M_at 403.5 P 0.000297
AFFX-BioB-3_at 197.8 P 0.000857
AFFX-BioC-5_at 558.1 P 0.000169
AFFX-BioC-3_at 492.7 P 0.000052
AFFX-BioDn-5_at 567.4 P 0.000044
AFFX-BioDn-3_at 3325.7 P 0.000169
AFFX-CreX-5_at 8523.2 P 0.000052
AFFX-CreX-3_at 8931.3 P 0.000044
AFFX-DapX-5_at 16.6 A 0.262809
AFFX-DapX-M_at 35.1 A 0.250796
AFFX-DapX-3_at 48.2 A 0.18511
AFFX-LysX-5_at 2.2 A 0.824672
AFFX-LysX-M_at 35.6 A 0.5
AFFX-LysX-3_at 20 A 0.131361
AFFX-PheX-5_at 1613.8 P 0.000044
AFFX-PheX-M_at 2060.1 P 0.000052
AFFX-PheX-3_at 3860.8 P 0.000044
AFFX-ThrX-5_at 10.7 A 0.659339
AFFX-ThrX-M_at 17.3 A 0.544587

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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