|
| Status |
Public on Feb 24, 2011 |
| Title |
ArthSynovialFluid.D03.2 |
| Sample type |
RNA |
| |
|
| Source name |
Synovial fluid
|
| Organism |
Mus musculus |
| Characteristics |
age: 6 weeks
cell: Whole SF cells
genetic background: B6
time: D03
separation method: Dissection/trimming
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Enzo kit (biotin)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
ArthSynovialFluid.D03.2, Synovial fluid cells were isolated during dissection of ankles obtained from mice 3 days after initial injection of K/BxN serum. RNA was extracted with Trizol and amplified/labeled using the MessageAmp and Enzo kits.
|
| Data processing |
CEL files were RMA normalized using ProbesetSummarizeEngine with apt-1.10.0.
|
| |
|
| Submission date |
Feb 23, 2011 |
| Last update date |
Feb 24, 2011 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE27492 |
Deficiency of CXCR2, but Not Other Chemokine Receptors, Attenuates Autoantibody-Mediated Arthritis in a Murine Model |
|
