|
| Status |
Public on Jun 21, 2023 |
| Title |
P29_EB300, replicate 1, scRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
Hypothalamus
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: Mixed
tissue: Hypothalamus
strain: Sprague Dawley
age: 4 weeks old
Sex: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After collagenase-assisted tissue dissociation, single-cell suspensions were converted into individually barcoded cDNA libraries with the Single-Cell 3’ Chromium kit version 3 from 10X Genomics.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v3.0.1 (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger) using a custom reference made for genome Rnor6.0 and Ensembl 96 gene models following 10X's method for the pre-built mouse reference
Assembly: Ensembl Rnor6.0, version 96
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Dec 14, 2022 |
| Last update date |
Jun 21, 2023 |
| Contact name |
CheMyong Jay Ko |
| E-mail(s) |
jayko@illinois.edu
|
| Organization name |
University of Illinois at Urbana Champaign
|
| Department |
Comparative Biosciences
|
| Street address |
2001 S. Lincoln Ave.
|
| City |
Urbana |
| State/province |
Illinois |
| ZIP/Postal code |
61802 |
| Country |
USA |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE221002 |
Sterilization of female rats by single-injection of estrogen: proof-of-concept for a nonsurgical alternative |
|
| Relations |
| BioSample |
SAMN32236126 |
| SRA |
SRX18704234 |
