|
| Status |
Public on Apr 01, 2012 |
| Title |
Mouse liver, constitutively active IKK2, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Trangenic mouse, biol. Replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
genotype: constitutively active IKK2 transgenic
genetic background: C57BL/6 and NMRI mixed, backcrossed at least 4-times to C57BL/6 background
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified from frozen liver samples kept in RNAlater (Qiagen) by RNAeasy mini kit (Qiagen) according to manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Whole Transcript (WT) Sense Target Labeling Assay
|
| |
|
| Hybridization protocol |
according to Affymetrix Whole Transcript (WT) Sense Target Labeling Assay
|
| Scan protocol |
according to Affymetrix protocols using a GeneChip 3000 Scanner
|
| Description |
The liver samples were collected from transgenic mice as well as from control animals at the age of 4 weeks
|
| Data processing |
Affymetrix® Expression Console™ Software for normalization, BRB Array Tools for statistical analysis
|
| |
|
| Submission date |
Mar 22, 2011 |
| Last update date |
Apr 01, 2012 |
| Contact name |
Karlheinz Holzmann |
| E-mail(s) |
karlheinz.holzmann@uni-ulm.de
|
| Organization name |
Ulm University
|
| Department |
Medical Faculty
|
| Lab |
Genomics Core Facility
|
| Street address |
Helmholtzstreet 8/1
|
| City |
Ulm |
| ZIP/Postal code |
D-89081 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE28085 |
Expression data from the mouse liver with constitutively active NF-kB |
|
