|
| Status |
Public on Mar 29, 2011 |
| Title |
C2_cancer_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
prostate cancer tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: prostate
gender: male
age: 77
psa: 8.26
gleason: 8
|
| Treatment protocol |
Tissue are fast protected by Liquid nitrogen and then kept in -80℃. Frozen tissue are confirmed by HE staining.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using the mirVana RNA Isolation Kit (Applied Biosystem) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Following the Agilent products processing protocol.
|
| |
|
| Hybridization protocol |
Following the Agilent products processing protocol.
|
| Scan protocol |
Following the Agilent products processing protocol.
|
| Description |
Gene expression of prostate cancer
|
| Data processing |
Raw data are imported using the GeneSpring GX 11 software, then normalized under the setting of percentile shift normalization algorithm.
|
| |
|
| Submission date |
Mar 28, 2011 |
| Last update date |
Mar 29, 2011 |
| Contact name |
Yifeng Luo |
| E-mail(s) |
lyif@mail.sysu.edu.cn
|
| Organization name |
The First Affiliated Hospital of Sun Yat-sen University
|
| Department |
Institute of Respiratory Diseases of Sun Yat-sen University
|
| Lab |
Division of Pulmonary and Critical Care Medicine
|
| Street address |
58 Zhongshan 2nd Road
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE28204 |
mRNA expression profile in PCa And BPH from Chinese patients |
| GSE34933 |
Expression profiles in PCa And BPH from Chinese patients |
|
