|
| Status |
Public on Nov 15, 2023 |
| Title |
Small intestine T conventional cells rep2 |
| Sample type |
RNA |
| |
|
| Source name |
small intestine
|
| Organism |
Mus musculus |
| Characteristics |
Sex: mixed male and female
genotype: K14Aβb, FOXP3GFP
strain: C57B6
age: 7-14 weeks
tissue: small intestine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Spleen and small intestine were processed to single cell suspensions. Cells were antibody stained for Flourescence activated cell sorted. Viability dye and antibodies against CD4, CD8, TCRb, and B220 were utilized to sort live TCRb+CD4+CD8-B220-GFP+ Treg cells and TCRb+CD4+CD8-B220-GFP- T conventional cells. RNA was extracted with Trizol reagent, followed by clean-up and with QIAGEN RNeasy mini kit before labeling and microarray preparations. Each sample includes cells from multiple mice to reach sufficient intestinal Treg numbers for RNA isolation
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Small intestine T conventional cells rep2
K14_gut_Tcon
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Feb 27, 2023 |
| Last update date |
Nov 15, 2023 |
| Contact name |
Terri Laufer |
| E-mail(s) |
tlaufer@pennmedicine.upenn.edu
|
| Phone |
(215)573-2955
|
| Organization name |
University of Pennsylvania
|
| Lab |
276 John Morgan Building
|
| Street address |
3620 Hamilton Walk
|
| City |
Philadlephia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE226193 |
Microarray expression analysis depicting MHCII-dependent features of FOXP3-expressing CD4 T regulatory cells |
|
