|
| Status |
Public on Jul 13, 2011 |
| Title |
5-KO_RD3a |
| Sample type |
RNA |
| |
|
| Source name |
5-KO_RD3a
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Smad3-/-
strain: mixed strains of C57BL/6 and NIH Black Swiss females (Taconic)
treatment: regular diet
|
| Treatment protocol |
The generation of Smad3-/- mice have been described in Yang, X., et al. Targeted disruption of SMAD3 results in impaired mucosal immunity and diminished T cell responsiveness to TGF-beta. Embo J 18, 1280-1291 (1999). Mice were fed with high fat diet (55% high fat diet) and antibodies (IgG and Anti-TGF-beta) were injected through intra-peritoneal route three times in a week for 8 weeks.
|
| Growth protocol |
All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
RMA by software Partek
|
| |
|
| Submission date |
Apr 13, 2011 |
| Last update date |
Jul 13, 2011 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE28598 |
Protection from obesity and diabetes by blockade of TGF-beta/Smad3 signaling |
|
