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Status |
Public on Jul 13, 2011 |
Title |
9_1aIgG |
Sample type |
RNA |
|
|
Source name |
9_1aIgG
|
Organism |
Mus musculus |
Characteristics |
strain: C57J/B6 treatment: diet induced obese antibody: IgG catalog#: 13C4
|
Treatment protocol |
The generation of Smad3-/- mice have been described in Yang, X., et al. Targeted disruption of SMAD3 results in impaired mucosal immunity and diminished T cell responsiveness to TGF-beta. Embo J 18, 1280-1291 (1999). Mice were fed with high fat diet (55% high fat diet) and antibodies (IgG and Anti-TGF-beta) were injected through intra-peritoneal route three times in a week for 8 weeks.
|
Growth protocol |
All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Data processing |
RMA by software Partek
|
|
|
Submission date |
Apr 13, 2011 |
Last update date |
Jul 13, 2011 |
Contact name |
WeiPing Chen |
E-mail(s) |
weipingChen@niddk.nih.gov
|
Phone |
301-496-0175
|
Organization name |
NIDDK/NIH
|
Department |
GCL
|
Lab |
Genomics Core Lab
|
Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE28598 |
Protection from obesity and diabetes by blockade of TGF-beta/Smad3 signaling |
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