GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM71511 Query DataSets for GSM71511
Status Public on Dec 01, 2005
Title MCF7_OF_3
Sample type RNA
Source name MCF7, oligofectamine, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were exposed to oligofectamine transfection reagent and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Affymetrix Expression Analysis Technical Manual
Hybridization protocol Refer to the Affymetrix Expression Analysis Technical Manual
Scan protocol Refer to the Affymetrix Expression Analysis Technical Manual
Description MCF7 cells exposed to oligofectamine and grown in Hypoxia
Data processing Normalized using the GCRMA algorithm
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
Platform ID GPL570
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
VALUE Signal, normalized using the GCRMA algorithm

Data table
1007_s_at 3350.8396
1053_at 806.89954
117_at 39.849804
121_at 240.58713
1255_g_at 4.448382
1294_at 10.178041
1316_at 18.6633
1320_at 9.3635235
1405_i_at 11.308867
1431_at 6.547876
1438_at 175.3401
1487_at 373.45612
1494_f_at 11.364723
1552256_a_at 2701.2944
1552257_a_at 597.5281
1552258_at 5.5371437
1552261_at 7.5495768
1552263_at 15.752826
1552264_a_at 345.5033
1552266_at 12.305079

Total number of rows: 54675

Table truncated, full table size 1088 Kbytes.

Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap