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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 31, 2023 |
| Title |
Nucleus accumbens, MAN1 male, rep1 [Lib02_S2] |
| Sample type |
SRA |
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| Source name |
Nucleus accumbens
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| Organism |
Microtus ochrogaster |
| Characteristics |
tissue: Nucleus accumbens
treatment: MAN1
Sex: M
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| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen AllPrep DNA/RNA mini kit
NEBNext Ultra Directional RNA Library Prep Kit with mRNA magnetic isolation
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Description |
Danoff_GEO_processed.csv
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| Data processing |
TrimGalore: removal of auto-deteted Illumina adapters and trimming of low-quality ends up to a threshold of Q20
MultiQC: quality control
Kallisto: pseudoalignment and transcript quantification
Assembly: Microtus ochrogaster, MichOch v1.0, Ensembl release version 96
Supplementary files format and content: csv; raw counts
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| Submission date |
Apr 08, 2023 |
| Last update date |
Jul 31, 2023 |
| Contact name |
Joshua Danoff |
| E-mail(s) |
jsd5zs@virginia.edu
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| Organization name |
University of Virginia
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| Street address |
485 McCormick Road
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| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22903 |
| Country |
USA |
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| Platform ID |
GPL31149 |
| Series (1) |
| GSE229256 |
Male specific impact of parental care on gene expression in nucleus accumbens |
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| Relations |
| SRA |
SRX19911198 |
| BioSample |
SAMN34121766 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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