|
| Status |
Public on May 17, 2012 |
| Title |
LU427 |
| Sample type |
RNA |
| |
|
| Source name |
Lung tumor, adenocarcinoma, smoker
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung carcinoma
assay: LU427
osbin: 0
os: 11.89041096
age: 47
Sex: F
gexcluster_ac: AC2
smoking: smoker
tnm: T2bN0M0
Stage: IIA
ckit_ihc: 1
her2_ihc: 0
pakt_ihc: 0
pten_ihc: 0
egfr_ihc: 1
egfr_amp: 0
egfr_mut: 0
pik3ca_mut: 0
kras_mut: 1
histology: AC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from fresh frozen lung tumor tissue was extracted as described PMID:17334996. RNA concentrations were measured using a Nanodrop (NanoDrop Technologies). RNA integrity was determined by an Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Illumina®Totalprep-96RNA Amplification kit 4397949 RevB
|
| |
|
| Hybridization protocol |
Illumina hybridization protocol (Manuell 11322187 Revision A)
|
| Scan protocol |
Standard Illumina iScan scanning protocol
Scan setting file: Direct Hyb
|
| Data processing |
The data were normalised using quantile normalisation implemented in BASE, http://base.thep.lu.se/
|
| |
|
| Submission date |
May 02, 2011 |
| Last update date |
Oct 09, 2012 |
| Contact name |
Johan Staaf |
| Organization name |
SCIBLU - Swegene Centre for Integrative Biology at Lund University
|
| Street address |
Medicon Village
|
| City |
Lund |
| ZIP/Postal code |
SE-223 81 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE29016 |
Relation between smoking history and gene expression profiles in lung adenocarcinomas |
| GSE29066 |
Molecular profiling of primary lung carcinomas |
|
