|
Status |
Public on May 01, 2012 |
Title |
6-AAB8 |
Sample type |
RNA |
|
|
Source name |
AAB8
|
Organism |
Mus musculus |
Characteristics |
background strain: mixed background of C57BL/6, FVB, and 129 genotype/variation: Stat5ab+/null
|
Treatment protocol |
The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
|
Growth protocol |
All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
The sorted cell subpopulations
|
Data processing |
RMA by software Partek
|
|
|
Submission date |
May 04, 2011 |
Last update date |
May 01, 2012 |
Contact name |
WeiPing Chen |
E-mail(s) |
weipingChen@niddk.nih.gov
|
Phone |
301-496-0175
|
Organization name |
NIDDK/NIH
|
Department |
GCL
|
Lab |
Genomics Core Lab
|
Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE29045 |
Genetic modification of STAT5 levels reveals dose-dependent and isoform-specific requirement for cell lineage progression and differentiation. |
|