|
| Status |
Public on May 01, 2012 |
| Title |
12_959 |
| Sample type |
RNA |
| |
|
| Source name |
959
|
| Organism |
Mus musculus |
| Characteristics |
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5abnull/Stat5a-
|
| Treatment protocol |
The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
|
| Growth protocol |
All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
The sorted cell subpopulations
|
| Data processing |
RMA by software Partek
|
| |
|
| Submission date |
May 04, 2011 |
| Last update date |
May 01, 2012 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE29045 |
Genetic modification of STAT5 levels reveals dose-dependent and isoform-specific requirement for cell lineage progression and differentiation. |
|
