|
| Status |
Public on May 25, 2006 |
| Title |
OEC_Lp_RAE2.0 |
| Sample type |
RNA |
| |
|
| Source name |
Primary culture of Olfactory Ensheathing Cells ONL adapted to long-term culture
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: Wistar
Age: 21 days postnatal
Tissue of origin: ONL of both OBs
Cells: cultured in M3 medium
PDs: over 50
|
| Growth protocol |
M3 medium: DMEM, 3% FBS, 20ug/ml Bovine Pituitary Extract, 2uM Forskolin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL
|
| Label |
Affymetrix hybridization
|
| |
|
| Description |
ONL was dissected from the OBs and cells were dissociated and plated. M3 medium allowed OEC selection. OEC characterization was performed by immunocytochemistry employing typical OEC markers. Over 90% OEC purity was obtained. Cells were serially passed in vitro and cultures that had surpassed 50 population doublings were established as a population of OECs adapted to long-term culture.
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Sep 07, 2005 |
| Last update date |
May 25, 2006 |
| Contact name |
Erika Pastrana |
| E-mail(s) |
epastrana@cbm.uam.es
|
| Phone |
+34 91 497 8475
|
| Fax |
+34 91 497 4799
|
| Organization name |
Centro de Biologia Molecular Severo Ochoa
|
| Department |
Universidad Autonoma de Madrid
|
| Street address |
Cantoblanco
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE3258 |
Comparison of three populations of OECs |
|
