|
| Status |
Public on Aug 31, 2011 |
| Title |
534-9-59 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
TN+ ADC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age: 50y
smoker: yes
Stage: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
CY3
|
| Label protocol |
10ug amplified RNA(aRNA) from tumor cells was labeled indirectly with CY3 and the same amout of human reference RNA labeled with CY5
|
| |
|
| Channel 2 |
| Source name |
Universal reference RNA (URR)
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: pool of 10 human carcinoma cell lines, including colon, breast, brain, lung ovarian carcinoma cells as a controlling intra- and inter-experimental variation from Stratagene.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
CY5
|
| Label protocol |
10ug amplified RNA(aRNA) from tumor cells was labeled indirectly with CY3 and the same amout of human reference RNA labeled with CY5
|
| |
|
| |
| Hybridization protocol |
Dual hybridisation of tumor sample labeled with Cy3 in ch 1 and URR labeled with Cy5 in ch 2 onto cDNA microarray slide. After hybridization, slides were washed sequential.
|
| Scan protocol |
Scanned on a GSI Lumonics 4000 Scanner
|
| Description |
laser captue microdissected tumor epithelial cells RNA for ch1 and URR for ch2 co-hybridisation
|
| Data processing |
Images were quantified using and ScanArray software (PerkinElmer Life Science Inc., Boston, Massachusetts). GeneSpring 6.1(Agilent) software was used.
|
| |
|
| Submission date |
Jun 08, 2011 |
| Last update date |
Aug 31, 2011 |
| Contact name |
Jiangting Hu |
| E-mail(s) |
jianting.hu@ndcls.ox.ac.uk
|
| Phone |
+44 1865 221763
|
| Organization name |
University of Oxford
|
| Department |
NDCLS
|
| Lab |
CRUK Tumour Pathology
|
| Street address |
Headley Way
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL334 |
| Series (1) |
| GSE29827 |
Human lung ADC and SCC comparsion study |
|
