|
| Status |
Public on Sep 27, 2011 |
| Title |
B-CLL sample 8 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
CD5+CD19+ non-SP B-CLL
|
| Organism |
Homo sapiens |
| Characteristics |
tumor location: PBMC
sorting selection: non-SP
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Miltenyi Biotec SuperAmp extraction, amplification and purification
|
| Label |
Cy3
|
| Label protocol |
250 ng of each of the cDNAs were used as template for Cy3 and Cy5 labeling which was performed according to Miltenyi Biotec’s undisclosed protocol
|
| |
|
| Channel 2 |
| Source name |
CD5+CD19+ SP B-CLL
|
| Organism |
Homo sapiens |
| Characteristics |
tumor location: PBMC
sorting selection: SP
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Miltenyi Biotec SuperAmp extraction, amplification and purification
|
| Label |
Cy5
|
| Label protocol |
250 ng of each of the cDNAs were used as template for Cy3 and Cy5 labeling which was performed according to Miltenyi Biotec’s undisclosed protocol
|
| |
|
| |
| Hybridization protocol |
Hybridization performed per Miltenyi Biotec's standard MACS Molecular Agilent hybridization protocol
|
| Scan protocol |
Scanned on an Agilent G2505B scanner; Images were quantified using Agilent Feature Extraction Software (v.9.1)
|
| Data processing |
Agilent Feature Extraction Software (v.9.1) was used for background subtraction
|
| |
|
| Submission date |
Jun 22, 2011 |
| Last update date |
Sep 27, 2011 |
| Contact name |
Aaron E Foster |
| E-mail(s) |
afoster@bellicum.com
|
| Phone |
832-384-1125
|
| Organization name |
Bellicum Pharmaceuticals
|
| Department |
Product Discovery
|
| Street address |
2130 W Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE30171 |
B-cell chronic lymphocytic leukemia side population cells |
|
