|
| Status |
Public on Nov 28, 2011 |
| Title |
xenograft_control_time48_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
xenograft tumour
|
| Organism |
Homo sapiens |
| Characteristics |
xenograft: ALL-3
mouse strain: NOD/SCID
sentrix id: 4457260087_C
time: 48
treatment: vehicle
replicate: 2
|
| Treatment protocol |
When high level engraftment was achieved the mice were randomised to receive either dexamethasone 15 mg/kg or vehicle control IP. Mice were culled at 0 hours, 8 hours, 24 hours or 48 hours following treatment. The mice in the 48 hour groups received a second dose of dexamethasone or vehicle control at 24 hours.
|
| Growth protocol |
Cells from ALL-3, a glucocorticoid-sensitive xenograft, were inoculated by tail-vein injection into 28 NOD/SCID mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy mini kit (Qiagen, Hilden, Germany) and the RNA integrity verified (Agilent Bioanalyzer, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the TotalPrep RNA Amplification kit for Illumina arrays (Ambion, Austin, TX)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
tumour derived from vehicle-treated mouse, at 48 hr post treatment, replicate 2
|
| Data processing |
The sample probe profiles with no normalisation or background correction were exported from BeadStudio (version 3.0.14, Illumina, San Diego, CA). The data were pre-processed using variance stabilising transformation and robust spline normalisation in lumi, from R/Bioconductor
|
| |
|
| Submission date |
Jul 05, 2011 |
| Last update date |
Nov 28, 2011 |
| Contact name |
Vivek A Bhadri |
| E-mail(s) |
vbhadri@ccia.unsw.edu.au
|
| Organization name |
Children's Cancer Institute Australia for Medical Research
|
| Department |
Leukaemia Biology Program
|
| Street address |
Lowy Cancer Research Centre
|
| City |
Randwick |
| State/province |
NSW |
| ZIP/Postal code |
2031 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE30392 |
The NOD/SCID Xenograft Model Provides Clinically-Relevant Insights into Glucocorticoid-Induced Gene Expression in Childhood B-Cell Precursor Acute Lymphoblastic Leukemia |
|
