|
| Status |
Public on Jul 10, 2023 |
| Title |
cornea-resveratrol-day7-rep2 |
| Sample type |
SRA |
| |
|
| Source name |
cornea
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: cornea
genotype: Wistar
treatment: resveratrol i.p.
|
| Treatment protocol |
SD rats as donors and Wistar rats as recipients to establish allograft corneal transplantation models.Postoperative intraperitoneal injection with resveratrol solution or saline daily until 7 days postoperatively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Fibrous Tissue Mini Kit (QIAGEN, Duesseldorf, Germany) was used to extract RNA from the corneas.
The libraries were sequenced on an llumina Novaseq 6000 platform and 150 bp paired-end reads were generated.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Res2-B1005Oct
|
| Data processing |
PCA analysis were performed using R (v 3.2.0) to evaluate the biological duplication of samples.
Differential expression analysis was performed using the DESeq2.
Hierarchical cluster analysis of DEGs was performed using R (v 3.2.0) to demonstrate the expression pattern of genes in different groups and samples.
Assembly: mRatBN7.2
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Jul 06, 2023 |
| Last update date |
Jul 10, 2023 |
| Contact name |
Chenjia Xu |
| E-mail(s) |
xuchj1189@163.com, xuchj23@mail2.sysu.edu.cn
|
| Organization name |
Zhongshan Ophthalmic Center
|
| Street address |
Zhongshan Ophthalmic Center
|
| City |
Guangzhou |
| ZIP/Postal code |
510060 |
| Country |
China |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE236626 |
Resveratrol prevents corneal allograft rejection in the rat cornea |
|
| Relations |
| BioSample |
SAMN36340900 |
| SRA |
SRX20924899 |
