animals were housed in Brown University's accredited animal care facility
Extracted molecule
total RNA
Extraction protocol
RNA trizol
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol
10 ucg of c RNA were hybridized for 17 hours at 45 degrees Celsius, 60rpm, FS450_0007
Scan protocol
Gene chips were scanned using the Affymetrix 7G3000 scanner
