|
| Status |
Public on Aug 30, 2011 |
| Title |
cbp+/- standard cage Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampal tissue from cbp+/- mice (standard cage)
|
| Organism |
Mus musculus |
| Characteristics |
strain background: F2 of C57BL/6J and DBA
gender: female
genotype/variation: cbp+/- (CBP deficient, heterozygous)
age: 3 months
tissue: hippocampus
|
| Treatment protocol |
Experiments were performed in 2-3 month old animals and in all cases the mice used as control were littermates of the mutant mice. Standard housing consisted of 30x15x11 cm clear cages occupied by up to 5 mice. The environmental enrichment boxes were large white plexiglass boxes (>1m2) and were occupied by a maximum of 20 mice. We used natural materials, plastic tubing, running wheels and toys to create an enriched environment whose configuration was modified every 48h shortly before the start of the dark cycle. The samples were obtained at least 16 h afther the last modification in the enviromental enrichment box to prevent any interference between the response to novelty and to environmental enrichment. The brains were immediately removed, and hippocampi rapidly dissected and placed in RNAlater solution (Qiagen, Venlo, The Netherlands) until total RNA extraction.
|
| Growth protocol |
CBP deficient heterozygous mice were maintained in a mixed background (F2 of C57BL/6J and DBA) (Alarcon et al., 2004). Mice were group-housed in single-sex cages on a light:dark cycle (12/12 h) with food and water available ad limitum. The wild type mice used as control were, in all cases, littermates of the mutant mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted separately from individual hippocampi using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) per manufacturers instructions followed by DNaseI treatment (Qiagen, Valencia, CA, USA) and a clean-up step using RNeasy RNA purification columns (Qiagen, Valencia, CA, USA). Equal amounts of total RNA from four animals were pooled, processed, and hybridized to GeneChIP Mouse Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA) according to the manufacture's protocol. Three to five independent biological replicates were prepared for each genotype and condtition (wild-type and crebbp deficient mice (cbp+/-; standar housing and environmental enrichment). GeneChIP® Mouse Gene 1.0 ST Arrays were hybridized, stained, washed and screened for quality according to the manufacturer's protocol. Microarray data were processed, normalized and statistically analyzed using GeneSpring GX (Agilent Technologies, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Following Affymetrix instructions
|
| |
|
| Hybridization protocol |
Following Affymetrix instructions
|
| Scan protocol |
Following Affymetrix instructions
|
| Description |
CBP_SC_3
Gene expression data from hippocampal tissue
|
| Data processing |
GeneSpring GX software (Agilent Technologies Inc) was used. RMA algorithm was uset to background correction, data normalization and probe sumarization.
|
| |
|
| Submission date |
Jul 22, 2011 |
| Last update date |
Sep 16, 2019 |
| Contact name |
Angel Barco |
| Organization name |
Instituto de Neurociencias (UMH-CSIC)
|
| Street address |
Av. Santiago Ramón y Cajal
|
| City |
Sant Joan d'Alacant |
| State/province |
Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE30880 |
CBP is required for environmental enrichment-induced neurogenesis and cognitive enhancement. |
|
