|
| Status |
Public on Jan 29, 2015 |
| Title |
HDLM-2_Perifosine 10µM_24hrs_2A |
| Sample type |
RNA |
| |
|
| Source name |
HDLM-2_Perifosine 10µM_24hrs
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HDLM-2
cell type: Human Hodgkin Lymphoma
treatment: 10 µM Perifosine for 24hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were processed and normalised using quantile normalisation with BeadStudio software
|
| |
|
| Submission date |
Jul 29, 2011 |
| Last update date |
Jan 29, 2015 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
|
| Organization name |
IRCSS Istituto Nazionale Tumori
|
| Street address |
via Venezian 1
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE31057 |
Gene expression profiling of HDLM-2 Hodgkin lymphoma cell line after in vitro and in vivo treatment with perifosine in combination with sorafenib |
| GSE31060 |
Gene expression analysis of Hodgkin lymphoma cell lines treated with the AKT inhibitor perifosine and the multikinase inhibitor sorafenib |
|
