|
| Status |
Public on May 24, 2024 |
| Title |
CD4Tcells-HFD-16hrstim-1 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells, sorted, spleen/Lymph node
|
| Organism |
Mus musculus |
| Characteristics |
agent: aCD3/CD28
tissue: Spleen/Lymph node
diet: High-Fat diet
strain: C57BL6
|
| Treatment protocol |
Cells were plated on TC-treated 96 well plates and stimulated with 2ug/ml plate-bound anti-CD3 and anti-CD28 antibodies for 16hrs.
|
| Growth protocol |
Naïve CD4+ T cells (CD4+ CD44low-int) were magnetically isolated by negative selection from spleens and lymph nodes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was then isolated (RNEAsy, Qiagen). Total RNA quality was analyzed by RNA 6000 Pico LabChip on a 2100 BioAnalyzer (Agilent).
|
| Label |
Biotin
|
| Label protocol |
Biotin Allonamide Triphosphate, performed by SickKids Microarray facility, Toronto, Canada
|
| |
|
| Hybridization protocol |
Each sample was hybridized to a GeneChip Mouse Gene 2.0 ST array (Affymetrix) for 16- 18 h at 45 °C
|
| Scan protocol |
The arrays were scanned using a GeneChip Scanner 3000 (Affymetrix)
|
| Description |
Gene expression data from sorted naïve CD4+ T cells from mice fed NCD or HFD for 4 weeks
|
| Data processing |
Data were analyzed using GeneSpring software (Version 12.1, Agilent) and was summarized using the ExonRMA16 algorithm followed by quantile normalization.
|
| |
|
| Submission date |
Aug 17, 2023 |
| Last update date |
May 24, 2024 |
| Contact name |
Brendan Cordeiro |
| E-mail(s) |
brendan.cordeiro@unityhealth.to
|
| Organization name |
St-michael's hospital
|
| Department |
Keenan Research Center
|
| Lab |
Dr. Shannon Dunn
|
| Street address |
209 Victoria St.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5B 1W8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE241044 |
Microarray data, 4 week HFD/NCD, nostim or 16hrstim |
|
