|
| Status |
Public on Nov 16, 2012 |
| Title |
Donor # 340 from monocytes deriving from apheresis # 1 manufacture 2 hybridized on slide # 5729 position 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mature Dendritic Cells of Donor
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: mature Dendritic Cells
donor: 340
apheresis: 1
manufacture: 2
slide #: 5729
position # on slide: 2
|
| Growth protocol |
Monocytes were cultured with GMCSF (1000 IU/ml) and IL4 (1000 IU/ml) for 3 days to obtain immature dendritic cells. To induce maturation LPS (30 ng/ml) and IFNg (1000 IU/ml) were added to the culture and cell were harvested 24 h later
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy kit from Qiagen
|
| Label |
Cy5
|
| Label protocol |
Test RNA and Universal Reference RNA () were amplified and labeled according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
| |
|
| Channel 2 |
| Source name |
Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: reference
vendor: Stratagene Catalog #740000
|
| Growth protocol |
Monocytes were cultured with GMCSF (1000 IU/ml) and IL4 (1000 IU/ml) for 3 days to obtain immature dendritic cells. To induce maturation LPS (30 ng/ml) and IFNg (1000 IU/ml) were added to the culture and cell were harvested 24 h later
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy kit from Qiagen
|
| Label |
Cy3
|
| Label protocol |
Test RNA and Universal Reference RNA () were amplified and labeled according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
| |
|
| |
| Hybridization protocol |
Samples were hybridized according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
| Scan protocol |
Scanned on an Agilent G2565BA scanner.
Images were quantified using Agilent Feature Extraction Software
|
| Description |
mature Dendritic Cells of Donor # 340 from monocytes deriving from apheresis # 1 manufacture 2 hybridized on slide # 5729 position 2
|
| Data processing |
Agilent Feature Extraction Software was used with dafault settings for processed signals
|
| |
|
| Submission date |
Aug 03, 2011 |
| Last update date |
Nov 17, 2012 |
| Contact name |
Luciano Castiello |
| E-mail(s) |
castiellol@cc.nih.gov
|
| Phone |
3014356380
|
| Fax |
3014358643
|
| Organization name |
NIH/CC
|
| Department |
DTM
|
| Lab |
CPS
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE31195 |
Study of the consistency, comparability and identity of different Dendritic cells productions for clinical use |
|
