|
Status |
Public on Nov 16, 2012 |
Title |
Donor # 340 from monocytes deriving from apheresis # 1 manufacture 2 hybridized on slide # 5729 position 4 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
mature Dendritic Cells of Donor
|
Organism |
Homo sapiens |
Characteristics |
cell type: mature Dendritic Cells donor: 340 apheresis: 1 manufacture: 2 slide #: 5729 position # on slide: 4
|
Growth protocol |
Monocytes were cultured with GMCSF (1000 IU/ml) and IL4 (1000 IU/ml) for 3 days to obtain immature dendritic cells. To induce maturation LPS (30 ng/ml) and IFNg (1000 IU/ml) were added to the culture and cell were harvested 24 h later
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using miRNeasy kit from Qiagen
|
Label |
Cy5
|
Label protocol |
Test RNA and Universal Reference RNA () were amplified and labeled according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
|
|
Channel 2 |
Source name |
Universal Human Reference RNA
|
Organism |
Homo sapiens |
Characteristics |
sample type: reference vendor: Stratagene Catalog #740000
|
Growth protocol |
Monocytes were cultured with GMCSF (1000 IU/ml) and IL4 (1000 IU/ml) for 3 days to obtain immature dendritic cells. To induce maturation LPS (30 ng/ml) and IFNg (1000 IU/ml) were added to the culture and cell were harvested 24 h later
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using miRNeasy kit from Qiagen
|
Label |
Cy3
|
Label protocol |
Test RNA and Universal Reference RNA () were amplified and labeled according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
|
|
|
Hybridization protocol |
Samples were hybridized according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
Scan protocol |
Scanned on an Agilent G2565BA scanner. Images were quantified using Agilent Feature Extraction Software
|
Description |
mature Dendritic Cells of Donor # 340 from monocytes deriving from apheresis # 1 manufacture 2 hybridized on slide # 5729 position 4
|
Data processing |
Agilent Feature Extraction Software was used with dafault settings for processed signals
|
|
|
Submission date |
Aug 03, 2011 |
Last update date |
Nov 17, 2012 |
Contact name |
Luciano Castiello |
E-mail(s) |
castiellol@cc.nih.gov
|
Phone |
3014356380
|
Fax |
3014358643
|
Organization name |
NIH/CC
|
Department |
DTM
|
Lab |
CPS
|
Street address |
10 Center Drive
|
City |
Bethesda |
State/province |
Maryland |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE31195 |
Study of the consistency, comparability and identity of different Dendritic cells productions for clinical use |
|