|
| Status |
Public on Jan 17, 2024 |
| Title |
PC3 miR-CON |
| Sample type |
RNA |
| |
|
| Source name |
Prostate cancer cell line, PC3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC3
cell type: Prostate cancer cell line
genotype: stably transfected with miR-410
|
| Treatment protocol |
miRNA vector (pMIR) with CMV promoter-driven expression of human pre-miRNA hsa-miR-410-GFP reporter and the corresponding scrambled construct were stably transfected into PC3 and C42B cell lines followed by puromycin selection.
|
| Growth protocol |
Cell lines stably expressing miR-CON/miR-410 were grown in RPMI media with 1 microgram/ml puromycin till logarithmic phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted using miRNAeasy minikit (Qiagen) according to manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
RNA was used to generate cDNAs and cDNAs were biotin-labeled according to the GeneCHip WT Plus reagent kit protocol.
|
| |
|
| Hybridization protocol |
Labeled samples were hybridized to the Clariom S Human array (Applied Biosystems) for 16 hours. The arrays were washed and stained using Affymetrix GeneChip Fluidics Station 450 systems.
|
| Scan protocol |
The stained arrays were scanned on Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from PC3 cell line stably transfected with miR-410
|
| Data processing |
Data was obtained in CEL file and imported into Partek Genomic Suites version 6.6 (Partek) using the standard imprt tool with an RMA normalization. The differential expressions were calculated using ANOVA of Partek package and filtered with a p-value cut-off of 0.05.
|
| |
|
| Submission date |
Oct 13, 2023 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Sang-Ho Kwon |
| E-mail(s) |
kkwon@augusta.edu
|
| Phone |
706-721-0379
|
| Organization name |
Augusta Univeristy
|
| Department |
Cellular Biology and Anatomy
|
| Street address |
1120 15th Street
|
| City |
Augusta |
| State/province |
Georgia |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE245308 |
Analyses of potential miR-410 target genes |
|
