|
| Status |
Public on Aug 01, 2012 |
| Title |
TC-1+MSCV retrovirus – Influenza 24 h rep1 |
| Sample type |
RNA |
| |
|
| Source name |
TC-1+MSCV retrovirus – Influenza 24 h
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
cell line: CRL-2785
cell type: Type I lung epithelial cell
|
| Growth protocol |
cells were cultured in DMEM + 10% heat-inactivated FBS + 2 mM L-glutamine + penicillin and streptomycin in the presence of 2 ug/mL puromycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were labeled according to the Affymetrix Whole Transcript Sense Target Labeling Assay, including the RiboMinus rRNA depletion step
|
| |
|
| Hybridization protocol |
Labeled, fragmented cDNA was hybridized for 16 hours at 45 deg C and 60rpm to a Affymetrix GeneChip. Washing was performed on the Fluidics with protocol FS450_0001.
|
| Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
| Description |
TC-1 cell line (CRL-2785) MSCV retrovirus – Influenza
|
| Data processing |
RMA normalization was done using the Affymetrix Power Tools (APT) software package.
|
| |
|
| Submission date |
Sep 07, 2011 |
| Last update date |
Aug 01, 2012 |
| Contact name |
madhumati gundapuneni |
| Organization name |
Institute for systems biology
|
| Street address |
401 Terry avenue North
|
| City |
seattle |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (2) |
| GSE31955 |
Effect of miR-144/miR451 expression on TC-1 lung epithelial cell responses to influenza infection for 24 hours [Expression] |
| GSE31957 |
Effect of miR-144/miR451 expression on TC-1 lung epithelial cell responses to influenza infection for 24 hours |
|
