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Sample GSM796351 Query DataSets for GSM796351
Status Public on May 16, 2012
Title PC12_NGF_24h
Sample type RNA
 
Source name PC12, 24h, 100 ng/ml NGF
Organism Rattus norvegicus
Characteristics cell line: PC12
cell type: pheochromocytoma
Treatment protocol Cells seeded on PolyD-lysine-coated plates were treated with 100 ng/ml murine NGF-7S (Invitrogen) in DMEM containing 1% HS and 0.5% FBS for 0, 12, 24 and 48 hr.
Growth protocol Rat pheochromocytoma PC12 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% horse serum (HS, Invitrogen, Carlsbad, CA, USA), 5% fetal bovine serum (FBS, MP Biomedicals, Irvine, CA, USA), 100 U/mL penicillin and 100 μg/mL streptomycin at 37ºC under a humidified atmosphere of 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA containing miRNA was extracted from cells using the miRNeasy Mini Kit (Qiagen, Hilden, Germany).
Label Cy3
Label protocol One hundred nanograms of total RNA was labeled using the Agilent miRNA Complete Labeling and Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions.
 
Hybridization protocol The hybridization used miRNA Complete Labeling and Hyb Kit according to the manufacturer's instructions.
Scan protocol After hybridization, the microarrays were washed according to the manufacturer’s protocol and scanned on an Agilent DNA Microarray Scanner with the Scan Control software (Agilent Technologies).
Description miRNA expression after 24hr in NGF-treated PC12
Data processing The resulting images were processed, and raw data were collected using Agilent’s Feature Extraction software. The gene expression data were analyzed using GeneSpring GX 11 (Agilent). The signal intensity for each probe was normalized by a percentile shift, in which each measurement was divided by the 75th percentile of all measurements in its array.
 
Submission date Sep 14, 2011
Last update date May 16, 2012
Contact name Toshio Kojima
Organization name Toyohashi University of Technology
Department Health Care Center
Street address 1-1 Hibarigaoka Tenpaku-cho
City Toyohashi
ZIP/Postal code 441-8580
Country Japan
 
Platform ID GPL10906
Series (1)
GSE32122 MicroRNA expression profiling of NGF-treated PC12 cells revealed a critical role for miR-221 in neuronal differentiation

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
rno-let-7a 3.3282785
rno-let-7b 2.8610802
rno-let-7b* -12.1115
rno-let-7c 3.2651472
rno-let-7d 2.2454662
rno-let-7d* -12.1115
rno-let-7e 1.3658094
rno-let-7e* -12.1115
rno-let-7f 3.2167196
rno-let-7i 2.492919
rno-let-7i* -12.1115
rno-miR-1 -12.1115
rno-miR-1* -12.1115
rno-miR-100 -12.1115
rno-miR-101a -3.7223206
rno-miR-101a* -12.1115
rno-miR-101b -2.8779445
rno-miR-103 0.37240887
rno-miR-106b -0.025632858
rno-miR-106b* -12.1115

Total number of rows: 350

Table truncated, full table size 7 Kbytes.




Supplementary file Size Download File type/resource
GSM796351_24h.txt.gz 398.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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