|
| Status |
Public on Apr 06, 2012 |
| Title |
Ly-6Clo monocyte, steady state - sample 5 |
| Sample type |
RNA |
| |
|
| Source name |
monocyte
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
gender: female
strain: C57BL/6
age: 6-8 weeks
cell type: monocyte
monocyte subset: Ly-6Clo
|
| Treatment protocol |
Spleens were homogenized in ice cold PBS with 0.5% BSA and 1mM EDTA, stained with fluorophore conjugated monoclonal antibodies and sorted directly in RLT lysis buffer on a FACS Aria (BD Biosystems). A negative selection (lineage depletion step) on a MACS LD magnetic column (Miltenyi) preceeded the sort. Total processing time <2h.
|
| Growth protocol |
6-8 wk old C57BL/6 mice, on regular chow diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the miRVANA microRNA isolation kit (Ambion).
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina label protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scan protocol.
|
| Description |
Ly-6Clo 2
|
| Data processing |
Raw data were exported and normalized using Gene Pattern 3.3.2, Build ID 9695, IlluminaNormalizer (normalization method: quantile)
|
| |
|
| Submission date |
Sep 26, 2011 |
| Last update date |
Apr 06, 2012 |
| Contact name |
Martin Etzrodt |
| E-mail(s) |
ETZRODT.MARTIN@MGH.HARVARD.EDU
|
| Phone |
(617) 643-0500
|
| Fax |
(617) 643-6133
|
| URL |
http://csb.mgh.harvard.edu/pittet
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Systems Biology
|
| Lab |
Pittet Lab
|
| Street address |
185 Cambridge Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL8180 |
| Series (2) |
| GSE32370 |
Regulation of monocyte functional heterogeneity by miR-146a |
| GSE32392 |
Regulation of Monocyte Functional Heterogeneity by miR-146a |
|
