|
| Status |
Public on Aug 31, 2012 |
| Title |
Pool_KO_Sample_17_09_08_14_15_16_11_12_18 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus, CA1 subfield KO
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: hippocampus, CA1 subfield
gender: male
genotype: ko_Mecp2-/y
average age (days after birth): 47.2
|
| Growth protocol |
animals were housed at the central animal facility of the University Medical Center Göttingen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was isolated from acute hippocampal tissue slices after microdissection of the CA1 subfield. Tissue samples were transferred into trizol buffer, homogenized and mRNA was isolated in a single-step acid guanidinium thiocyanate-phenol-chloroform extraction following manufacturer´s instructions (http://tools.invitrogen.com/content/sfs/manuals/trizol_reagent.pdf)
|
| Label |
Cy3
|
| Label protocol |
For each array 200 ng RNA was processed and Cy3 labeled by the Low Input Quick Amp Labeling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Hybridization was performed in the hybridization oven (Agilent Technologies, 17h, 10 rpm, 65˚C)
|
| Scan protocol |
Cy3 intensities were detected by a one-color microarray scanner (G2505C, Agilent Technologies) at 5 µm resolution
|
| Description |
mRNA hippocampus, CA1 subfield, adult mouse, pooled mRNA of all 9 KO animals
|
| Data processing |
Data were quantile normalized and log2 transformed using R (2.13.0) and Bioconductor package limma (3.8.2)
|
| |
|
| Submission date |
Oct 11, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Human Genetics
|
| Lab |
NGS Integrative Genomics
|
| Street address |
Justus-von-Liebig-Weg 11
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37077 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10333 |
| Series (1) |
| GSE32870 |
Oxidative burden and mitochondrial dysfunction in a mouse model of Rett syndrome |
|
