|
| Status |
Public on Jan 13, 2012 |
| Title |
IgG_IMR90_ChIPseq |
| Sample type |
SRA |
| |
|
| Source name |
human IMR90 fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IMR90
cell type: fetal lung fibroblast
antibody: IgG
|
| Growth protocol |
Minimum Essential Medium, Eagle with Earle's Balanced Salt Solution supplemented with 10% Fetal Bovine Serum (FBS), 1% Pen/Strep (penicillin, streptomycin), 1% GlutaMax (stable form of glutamine)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
See the library protocol linked to the Series record.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
Following sequencing cluster imaging, base calling were conducted using the Illumina pipeline. Reads were mapped to human hg18 with Illumina's Eland package.
|
| |
|
| Submission date |
Oct 20, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Ulrich Wagner |
| Organization name |
Uiversity Hospital Zurich
|
| Street address |
Schmelzbergstrasse 12
|
| City |
Zurich |
| ZIP/Postal code |
8091 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL10999 |
| Series (1) |
| GSE33128 |
Regulation of Mediator-dependent transcriptional activation of Pol II(G) through TFII |
|
| Relations |
| Reanalyzed by |
GSE35878 |
| SRA |
SRX101772 |
| BioSample |
SAMN00740010 |
